Sieve 2.0

A new workflow to overcome analytical and informatics challenges in metabolomics has been developed by Thermo Fisher Scientific. The workflow integrates new application software

Thermo Scientific's Sieve 2.0 differential expression analysis and Mass Frontier 7.0 structural elucidation software

with powerful new Thermo Scientific high-resolution accurate mass  instruments: the Q Exactive high performance benchtop quadrupole-Orbitrap LC-MS/MS and the Orbitrap Elite hybrid mass spectrometer. The new products accelerate even the most complex metabolomics studies and were unveiled at the 7th International Conference of the Metabolomics Society, at the end of June, in Cairns, Australia.

'Progress in metabolomics has been severely impeded by data-processing bottlenecks,' said Dr Mark Sanders, director of the demonstration and application laboratories of Thermo Fisher Scientific in North America. 'The new capability in Sieve 2.0 software eliminates the most significant bottlenecks in current metabolomics data-processing, enabling scientists to find the most promising putative biomarkers quickly.'

Sieve software is an automated solution for label-free, semi-quantitative differential expression analysis of proteins, peptides and metabolites. Using the software to pre-filter data generated by mass spectrometers greatly reduces the number of compounds that need to be identified, increasing the throughput of complex biomarker discovery experiments significantly.

The new features include:

  • Advanced background subtraction removes chemical noise, eliminating false positives and increasing confidence in results.
  • Automated spectral interpretation simplifies the data obtained from LC-MS experiments.
  • Streamlined data analyses and interpretation facilitate identification of the most promising putative components.
  • New statistical and visualisation tools speed-up the analysis of differentially expressed components.

For functionality and security for externalised research, software providers have turned to the cloud, writes Sophia Ktori


Robert Roe looks at the latest simulation techniques used in the design of industrial and commercial vehicles


Robert Roe investigates the growth in cloud technology which is being driven by scientific, engineering and HPC workflows through application specific hardware


Robert Roe learns that the NASA advanced supercomputing division (NAS) is optimising energy efficiency and water usage to maximise the facility’s potential to deliver computing services to its user community


Robert Roe investigates the use of technologies in HPC that could help shape the design of future supercomputers