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Earlham Institute projects shed light on the wheat genome

The Earlham Institute (EI) has announced new research collaborations and software tools to facilitate a better understanding of wheat genomes.

EI released details of five new wheat genome assemblies that EI presented publicly at PAG, the largest plant and animal genomics global conference, in San Diego, on 14 January.

This new analysis was possible thanks to a method that uses a bioinformatics tool ‘w2rap’ tool for genome assembly, developed by Bernardo Clavijo and his Algorithm Development Team at EI.

W2rap is a bioinformatics pipeline that can decipher complex genomes and produce assemblies to guide precision breeding. This genome assembly method will be applied to EI’s further wheat analysis, using tailored data generation based on work of the Institute’s Platforms and Pipelines Group at EI.

Bernardo Clavijo, bioinformatics algorithms project leader, said: ‘w2rap will be of great value for public wheat initiatives; helping us to advance into wheat pan-genomics faster, and from a completely open and transparent public-research focus. From a breeder’s perspective, this is also a tool that allows them to peek inside their germplasm with unprecedented detail and robustness - allowing cross-analysis with our high-quality public wheat reference. We are thrilled to be working with Bayer Crop Sciences; making the impact of powerful whole-genome sequencing and assembly approaches a reality.

After the initial development of the method and the release of a single wheat genome Bayer Crop Sciences approached the team to run a pilot project sequencing a commercially significant wheat variety.

Fred Van Ex, head of the genomics group at Bayer Crop Science, said: ‘More than two billion people worldwide rely on wheat as a staple food. In order to meet demands of the growing world population, Bayer is dedicated to develop high-yielding wheat plants that are able to withstand increasing environmental stresses and provide high-quality end products. Additional wheat genome sequences will allow us to accelerate the identification of genes underlying yield and resistance to biotic and abiotic stress. We are, therefore, very excited to work with EI as the wheat genome sequences generated by the w2rap pipeline will enable us to develop markers for breeding and isolate genes to support the development of improved wheat varieties that meet the farmers and society needs.’

The tool will not only be able to assemble the wheat varieties sequence data but allow wheat genomes to be produced effectively, robustly and efficiently with next-generation sequencing technologies.

‘In terms of global food security, the gains are twofold. The immediate incorporation of new technology into the breeding programme allows breeders to produce better varieties faster and cheaper. As well as the methods we develop enabling a fast transition from a single-reference to a many-genomes scenario that will effectively start to tap in the enormous variation of the world’s wheat genomic resources’ said Clavijo.

In parallel to this, a £2m grant funded by BBSRC to EI in collaboration with the John Innes Centre (JIC) and NIAB, led by Dr Matt Clark, will aim to understand the genetic makeup of 14 different varieties of wheat cultivars important for global agriculture and analyse this data to help map the genetic variation in the crop. This project includes the further sequencing and assembly of eight more wheat lines with the w2rap method, and will build wheat genetic resources for the purpose of crop breeding, preservation, and research.

Prof Neil Hall, director of EI, said: ‘These new projects are extremely timely as technical advances we have made in assembly and analysis of the wheat genome have overcome the hurdles in complexity and now enable us to approach wheat genetics in a whole new way. Now we should see the rapid advances in breeding programs that have previously been possible in other crops.’

Grant lead Dr Matt Clark, head of technology development at EI, said: ‘These new wheat genomes sequences have the potential to radically alter both wheat breeding and crop improvement. Facilitating new areas of research in understanding the contributions of genetic variation and gene function to the crop’s many different traits.'

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